Lab Report of the Experiment of Conjugation of E. Coli

Abstract:Conjugation is a natural drawring process that involves the tape drive of deoxyribonucleic acid from 1 electric cadre into another by means of a tangible connection between the cells. In the succeeding(a) experiment, deuce gos of Esche enoughia coli bacteriuml cells ( giver F?lac+strs and receiver F-lac-strr) underwent conjugation to produce a transconjugant strain (F?lac+strr). MAC exfoliations and streptomycin were utilized to determine if conjugation had occur expiration. When plated, the sponsor colonies appeared red and the recipient colonies appeared white. The transconjugant plates showed red and white colonies. maturation alkaline lysis miniprep, a DNA plasmid was isolated from the donor and transconjugant strains and FIGE electrophoresis was apply to determine the size of the plasmid. The conjugation ability was found to be 16.25% and the plasmid DNA was approximately 97 kilobases long. The results show that the F? plasmid was effectively tra nsferred from the donor cells into the recipient cells via conjugation. Introduction:bacterial conjugation is the unidirectional transfer of each genomic DNA or plasmid DNA from a donor bacterial cell to a recipient bacterial cell by cell-to-cell affect via a sex pilus (Snustad & amp; Simmons, 2006). Conjugation was for the first time discovered by Lederberg and Tatum in 1946. In their experiment, they grew two strains of bacteria in separate vessels with rich medium and then in concert in one vessel containing the same medium.

Then, they spread the ternion vessel table of contents onto medium agar plates and i ncubated them overnight at 37?C. The yet p! late that showed cell growth was the plate containing the mixture of the two bacterial strains. The other two plates showed no growth. This experiment proved that in order for recombination to occur, the two strains mustiness come in middleman with one another (Lederberg, Tatum, 1946). In 1950, Bernard Davis discovered that cell-to-cell contact was required to obtain a transconjugant. Using a U tube containing a sintered... If you want to fit a rich essay, order it on our website: BestEssayCheap.com

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